Cereal biotechnology by Peter C. Morris, James H. Bryce

By Peter C. Morris, James H. Bryce

(Woodhead Publishing restricted) Discusses genetic amendment in cereal biotechnology, explaining the root for present rules and strategies for assessing dangers. additionally addresses particular purposes for cereal biotechnology from using molecular organic instruments cereal creation tools.

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Furthermore, the development of this technology relies upon efficient protoplast-to-plant regeneration systems, which are often genotype dependent. The production of cell suspensions which release totipotent protoplasts is, theoretically, simple. However, in practice, it is laborious, time consuming, and relies heavily upon the intuition of the worker to identify and to select at an early stage of culture those cells which are most likely to produce cell suspensions suitable for protoplast isolation.

Pp. 113–22. ) using in vitro shoot meristematic cultures derived from germinating seedlings. Plant Cell Reports, 1999 18(12) 959– 66. ¨ LLER, B, SCHULZE, J, KOLESNIKOV, V and ZELENIN, A, MENDEL, R R, MU Delivery of foreign genes to intact barley cells by high-velocity microprojectiles. Theoretical Applied Genetics, 1989 78 31–4. 30. CHENG, M, FRY, J E, PANG, S, ZHOU, H, HIRONAKA, C M, DUNCAN, D R, CONNER, T W and WAN, Y, Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiology, 1997 115 971–80.

Before regeneration can take place, the transformed target tissue is maintained for a substantial time on selective medium which also contains auxins. The combination of auxins and selective agents favours the proliferation of transformed cells. The regeneration capacity of the transformed cells decreases usually with the period of tissue culture and the doses of the selectable agent and this has therefore to be minimised. The maintenance medium for barley has empirically been optimised by including for instance cytokinins.

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