By P.M. DEY (Eds.)
The sequence, Methods in Plant Biochemistry, presents an authoritative reference on present innovations within the numerous fields of plant biochemical learn. each one quantity within the sequence will, less than the professional assistance of a visitor editor, care for a specific crew of plant compounds. each one will describe the ancient heritage and present, most precious tools of research. The volumes comprise particular discussions of the protocols and suitability of every approach. Case remedies, diagrams, chemical buildings, reference information, and homes can be featured in addition to an entire record of references to the professional literature.
Conceived as a pragmatic significant other to The Biochemistry of crops, edited via P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can manage to pay for to be with out this accomplished and up to date reference resource
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After stirring the solution at 65°C for 10 min, insoluble material is removed by filtration and the soluble hexuronic acid salts are reduced to the corresponding aldonic acids by the addition of sodium borohydride (50 mg). After 1 h at room temperature the excess borohydride is destroyed by addition of a cation-exchange resin (H + form) (2 x 1 cm). The resin and solution are then added to a small (6 x 1 cm) column of the same resin, and aldonic acid is eluted with water. The combined eluate and washings are evaporated to dryness under reduced pressure.
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