Analytical Ultracentrifugation of Polymers and Nanoparticles by Walter Maechtle

By Walter Maechtle

This ebook is split into chapters overlaying instrumentation, sedimentation pace runs, density gradient runs, program examples and destiny advancements. particularly, the precise software bankruptcy demonstrates the flexibility and gear of AUC by way of many fascinating and significant business examples. therefore the ebook concentrates on sensible points instead of info of centrifugation concept.

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Then, the reciprocal of ρ, the specific volume v = 1/ρ, is plotted as function of c. The slope of a regression line yields the wanted value of ν¯ = (ρp )−1 of the solute. The example presented in Fig. 15 is a polybutyl acrylate latex dispersed in water at 25 ◦ C. 954cm3 /g. Another important possibility to determine the solute density ρp will be subject of Chap. 4: the determination of particle densities in AUC density gradients. The use of a pycnometer is also possible, but tedious. Furthermore, a classical method to determine solution densities is the use of calibrated sinkers/floaters that make use of the Archimedes principle.

Detectors can also be classified as follows (see Figs. 13): – Detectors that scan the cell radially (see Fig. 8d). , along the cell sectors. The step width is adjustable, and usually lies in the range 10–100 μm. At each radial position r, the optical information is recorded. The single measurements are then added to result in a radial concentration profile c(r) of the cell at given time t. The most important example for a scanning type of detector is the UV/Vis absorption detector, as it is realized in the Optima XL-A, and the known fluorescence detectors.

The measurements of the two most important AUC parameters, υ = 1/ρp and (dn/dc)p , are presented in the two following sections. 1 Measurement of the Solvent Density and the Partial Specific Volume As mentioned above, the partial specific volume ν¯ is the inverse of the solute/particle density ρp . The partial specific volume ν¯ is defined as the volume increase obtained if 1g of solute is added to an infinite amount of solvent. Precise knowledge 44 2 Analytical Ultracentrifugation, Instrumentation of this parameter is crucial for the interpretation of sedimentation data.

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